![]() The authors have declared no competing interest. We believe that it will become a powerful tool in gene annotation using closely related species for phytologists. GFAP contained large amount of plant-species information. The website of provides the software, databases, testing data and video tutorials for users. The LEADTOOLS sample apps are intended to show. This is one of the sample applications that is shipped with LEADTOOLS, the award-winning SDK for programmers. This application is built using the LEADTOOLS Image Annotation SDK. In addition, GFAP can also perform the sequence alignment, statistical analysis and drawing. The LEADTOOLS Annotations application can be used to create\view Annotations files. GFAP can search the protein domain, GO and KEGG information for 43000 genes within 4 minutes. GFAP, an open-source software running on Windows and MacOS systems, is an efficient and network independent tool. One of the nicest features of the interactive display is the multi-way whiteboarding. The board comes equipped annotation software, or you can connect it to your laptop using video and USB cables. Furthermore, we developed the software, Gene Annotation Software for Plants (GFAP), to perform gene annotation using our databases. To turn on the interactive display, you can use the power button on the side of the Interactive board or just grab one of the markers. If you are using Windows 10 or above, you find this cool annotation tool by default. This app is compatible with almost all operating. ![]() After that, these sequences were annotated by pfam, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases to construct our databases. Adobe Reader is probably the most used PDF reader. Here, we collected the protein sequences of 236 plant species with known genomic information from 63 families. Therefore, limited species number in previous software or website is disadvantageous for plant gene annotation. Selecting closely related species as the reference species should be an effective way to improve the accuracy of gene annotation for plants, compared with only based on one or some model plants. Sequence alignment is the basis of gene functional annotation for unknow sequences.
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